About ⅓ or a third of the way, you can stop at North Ruskin, FL. This is about half an hour south of Tampa.
About ⅔ or two thirds of the way, you can stop at Fullers Earth, FL. This is almost an hour south of Tampa, or about half an hour north of Sarasota.
If you drive straight through without stopping at all, the total distance is 60 miles. The distances shown above don't include local roads to get into each town, so if you're planning a long road trip, make sure you factor in traffic and road conditions, rest stop breaks, and interesting detours along the way. If you're in a rush, you can do the trip with zero stops, and your total travel time would be around 1 hour.