About ¼ or a fourth of the way, you can stop at Deltona, FL. This is about half an hour southwest of Daytona Beach.
About ⅓ or a third of the way, you can stop at Sanford, FL. This is about half an hour southwest of Daytona Beach.
About ⅔ or two thirds of the way, you can stop at Doctor Phillips, FL. This is a little over 1 hour southwest of Daytona Beach, or almost an hour northeast of Lakeland.
About ¾ or three fourths of the way, you can stop at Loughman, FL. This is about 1½ hours southwest of Daytona Beach, or about half an hour northeast of Lakeland.
If you drive straight through without stopping at all, the total distance is 112 miles. The distances shown above don't include local roads to get into each town, so if you're planning a long road trip, make sure you factor in traffic and road conditions, rest stop breaks, and interesting detours along the way. If you're in a rush, you can do the trip with zero stops, and your total travel time would be around 1 hour, 54 minutes.